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Neutrophil Extracellular Traps: How to Generate and Visualize Them
Chapters
- 00:00Title
- 00:47Introduction
- 01:32PMN Isolation from Human Blood
- 05:11Activating PMNs
- 06:46NET Detection by Immunolabeling
- 08:16Preparing NETs for Scanning Electron Microscopy (SEM)
- 12:16Representative Results
- 13:18Conclusion
Neutrophil Extracellular Traps (NETs) are an important innate immune mechanism to fight pathogenic bacteria, fungi and parasites. Here we describe methods to isolate neutrophil granulocytes from human blood and to activate them to form NETs. We present preparation techniques to visualize NETs in light and electron microscopy.
Tags
Neutrophil Extracellular TrapsNETsLeukocytesPhagocytesAntimicrobial GranulesGranule ProteinsChromatinPathogensCorrelative MicroscopyTEMSEMImmunofluorescenceLive Cell ImagingNucleiEu- And HeterochromatinNuclear EnvelopeGranule MembranesCell Death ProgramNETosisReactive Oxygen SpeciesNADPH OxidaseAcute InflammationInnate Immune Response
