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Stemgent İnsan TF lentivirüs İnsan Fibroblastlar yeniden programlanması Bağlı Pluripotent Kök Hücre Üretimi ayarla
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Biology
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JoVE Journal Biology
Generation of Induced Pluripotent Stem Cells by Reprogramming Human Fibroblasts with the Stemgent Human TF Lentivirus Set
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Stemgent İnsan TF lentivirüs İnsan Fibroblastlar yeniden programlanması Bağlı Pluripotent Kök Hücre Üretimi ayarla

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29,476 Views

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12:10 min

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December 08, 2009

DOI:

10.3791/1553-v

DOI:
10.3791/1553-v

•

12:10 min
December 08, 2009

•

12 Views
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1Research and Development,Stemgent

Summary

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Biz, insan faktörleri Oct4, Sox2, Nanog ve Lin28 lentivirüs aracılı teslim kullanarak insan somatik hücre alınan uyarılmış pluripotent kök hücreleri üretimi için protokol göstermektedir. Pluripotency morfolojisi ve embriyonik kök (ES) hücre-spesifik belirteçler varlığı teyit edilmiştir.

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Wu, D., Hamilton, B., Martin, C., Gao, Y., Ye, M., Yao, S. Generation of Induced Pluripotent Stem Cells by Reprogramming Human Fibroblasts with the Stemgent Human TF Lentivirus Set. J. Vis. Exp. (34), e1553, doi:10.3791/1553 (2009).

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