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İnsan Postmortem Beyin Dokusu Tek Hücre Popülasyonlar Yüksek Kalite RNA Alınması
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Biology
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JoVE Journal Biology
Obtaining High Quality RNA from Single Cell Populations in Human Postmortem Brain Tissue
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İnsan Postmortem Beyin Dokusu Tek Hücre Popülasyonlar Yüksek Kalite RNA Alınması

Türkçe

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30,342 Views

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18:17 min

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August 06, 2009

DOI:

10.3791/1444-v

DOI:
10.3791/1444-v

•

18:17 min
August 06, 2009

•

1 Views
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1Department of Structural and Molecular Neuroscience,McLean Hospital, 2Department of Psychiatry,Harvard Medical School, 3Department of Psychiatry,Beth Israel Deaconess Medical Center

Summary

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Biz postmortem insan beyni superior temporal girus III. Tabakası homojen bir hücre popülasyonu, piramidal nöronlar, RNA izole etmek ve ayıklamak için lazer yakalama mikrodiseksiyon kullanarak bir süreci tanımlar. Biz (T7 tabanlı) mRNA daha sonra doğrusal yükseltmek. Kullanmakta olduğunuz örnek Affymetrix insan X3P mikroarray melezleşme.

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Cite this Article

Pietersen, C. Y., Lim, M. P., Woo, T. W. Obtaining High Quality RNA from Single Cell Populations in Human Postmortem Brain Tissue. J. Vis. Exp. (30), e1444, doi:10.3791/1444 (2009).

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