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Obtaining High Quality RNA from Single Cell Populations in Human Postmortem Brain Tissue
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August 06, 2009
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We describe a process using laser-capture microdissection to isolate and extract RNA from a homogeneous cell population, pyramidal neurons, in layer III of the superior temporal gyrus in postmortem human brains. We subsequently linearly amplify (T7-based) mRNA, and hybridize the sample to the Affymetrix human X3P microarray.
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High Quality RNASingle Cell PopulationsGray Matter ReductionSuperior Temporal GyrusSchizophrenia PatientsGene Expression ProfilesPyramidal NeuronsCerebral CortexLaser-capture Microdissection (LCM)Histogene Staining SolutionArcturus XT LCM SystemT7-based Linear AmplificationExperion LabChip (Bio-Rad) GelElectropherogramMRNATranscript LengthMicroarraysHuman X3P GeneChip Probe ArrayAffymetrix
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Pietersen, C. Y., Lim, M. P., Woo, T. W. Obtaining High Quality RNA from Single Cell Populations in Human Postmortem Brain Tissue. J. Vis. Exp. (30), e1444, doi:10.3791/1444 (2009).
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