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June 29, 2009
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Details methods for high-resolution Ca2+ imaging of smooth muscle within isolated organs, including: preparation of the tissue, image acquisition and data analysis.
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Focal Ca2+ Transient DetectionSmooth MuscleCa2+ ImagingCellular MechanismsMembrane PotentialInternal StoresMultiple CellsCouplingSubcellular Ca2+ TransientsStochastic OccurrenceWide Field Of ViewContractionImaging ProtocolsAnalysis RoutinesFrequencyLocationAmplitudePurinergic Ca2+ TransientsTransmitter ReleaseSubmicron ResolutionATP ReleaseP2X1 ReceptorsDetrusorVas DeferensPurinergic Neuroeffector Ca2+ Transients (NCTs)Optical MonitoringNeurotransmitter ReleaseSpatial Resolution
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Young, J. S., Amos, R. J., Brain, K. L. Focal Ca2+ Transient Detection in Smooth Muscle. J. Vis. Exp. (28), e1247, doi:10.3791/1247 (2009).
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