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Focal Ca2+ Transient Detection in Smooth Muscle
JoVE Journal
Biologia
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JoVE Journal Biologia
Focal Ca2+ Transient Detection in Smooth Muscle

Focal Ca2+ Transient Detection in Smooth Muscle

DOI:
10.3791/1247-v

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17:41 min

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June 29, 2009

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, ,
1Department of Pharmacology,University of Oxford

Capitoli

  • 00:05Titolo
  • 00:56Introduction
  • 01:30Preparing the Ca2+ Indicator, Dissecting the Tissue, and Loading the Ca2+ Indicator
  • 05:05Preparing the ‘Ca2+ Loaded’ Tissue for Microscopy
  • 08:44Preparing for Analysis and Correcting for Movement
  • 11:57Particle Detection Algorithm
  • 16:53Conclusion

Summary

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Details methods for high-resolution Ca2+ imaging of smooth muscle within isolated organs, including: preparation of the tissue, image acquisition and data analysis.

Tags

Focal Ca2+ Transient DetectionSmooth MuscleCa2+ ImagingCellular MechanismsMembrane PotentialInternal StoresMultiple CellsCouplingSubcellular Ca2+ TransientsStochastic OccurrenceWide Field Of ViewContractionImaging ProtocolsAnalysis RoutinesFrequencyLocationAmplitudePurinergic Ca2+ TransientsTransmitter ReleaseSubmicron ResolutionATP ReleaseP2X1 ReceptorsDetrusorVas DeferensPurinergic Neuroeffector Ca2+ Transients (NCTs)Optical MonitoringNeurotransmitter ReleaseSpatial Resolution

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