Journal
/
Biology
/
The Preparation of Primary Hematopoietic Cell Cultures From Murine Bone Marrow for Electroporation
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
The Preparation of Primary Hematopoietic Cell Cultures From Murine Bone Marrow for Electroporation

The Preparation of Primary Hematopoietic Cell Cultures From Murine Bone Marrow for Electroporation

DOI:
10.3791/1026-v

•

08:15 min

•

January 06, 2009

•

, ,
1Gene Expression Division,Bio-Rad Laboratories, Inc

Chapters

  • 00:00Title
  • 00:08Introduction
  • 00:43Harvesting Bone Marrow From Femurs and Tibiae
  • 02:07Establishing Cultures
  • 04:33Electroporating Cells
  • 07:13Transfection Results
  • 07:53Conclusion

Summary

Automatic Translation

Automatic Translation

This procedure describes how to establish primary hematopoietic cell cultures from murine bone marrow and is followed by transfection using the Gene Pulser MXCell electroporation system.

Tags

Primary Hematopoietic Cell CulturesMurine Bone MarrowElectroporationPlasmid DNASiRNAGene Pulser MXcell Electroporation SystemGene Pulser Electroporation BufferTransfectionNucleic AcidsMammalian CellsDifficult-to-transfect CellsStem CellsVideo DemonstrationFemurTibia

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/1026?article_type=v

Derivation of Hematopoietic Stem Cells from Murine Embryonic Stem Cells
check_url/1026?article_type=v

Primary Neuronal Cultures from the Brains of Late Stage Drosophila Pupae
check_url/1026?article_type=v

Preparation of Neuronal Cultures from Midgastrula Stage Drosophila Embryos
check_url/1026?article_type=v

Electroporation of Mycobacteria
check_url/1026?article_type=v

Primary Dissociated Midbrain Dopamine Cell Cultures from Rodent Neonates
check_url/1026?article_type=v

Homing of Hematopoietic Cells to the Bone Marrow
check_url/1026?article_type=v

Primary Culture and Plasmid Electroporation of the Murine Organ of Corti.

check_url/1026?article_type=v

Primary Cell Cultures from Drosophila Gastrula Embryos
check_url/1026?article_type=v

Fremstilling af primær Myogenic precursorcelle / myoblast kulturer fra Basal hvirveldyr afstamninger
check_url/1026?article_type=v

Osteoclast Derivation fra Mouse Bone Marrow

Read Article