This protocol presents a transpupillary vision-guided trans-scleral approach to safely and precisely deliver subretinal cellular grafts, with a low rate of surgical complications, in mouse recipients with or without retinal degeneration.
Liu, Y. V., Li, K. V., Li, Z., Lu, Y., McNally, M. M., Esposito, E. P., Aziz, K., Singh, M. S. Transpupillary-Guided Trans-Scleral Transplantation of Subretinal Grafts in a Retinal Degeneration Mouse Model. J. Vis. Exp. (203), e65448, doi:10.3791/65448 (2024).