Transpupillary-Guided Trans-Scleral Transplantation of Subretinal Grafts in a Retinal Degeneration Mouse Model

Ying V. Liu; Kang V. Li; Zhuolin Li; Yuchen Lu; Minda M. McNally; Edward P. Esposito; Kanza Aziz; Mandeep S. Singh

doi:10.3791/65448

Journal

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Neurowissenschaften

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Transpupillary-Guided Trans-Scleral Transplantation of Subretinal Grafts in a Retinal Degeneration Mouse Model

JoVE Journal
Neurowissenschaften
Author Produced

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JoVE Journal Neurowissenschaften

Transpupillary-Guided Trans-Scleral Transplantation of Subretinal Grafts in a Retinal Degeneration Mouse Model

806 Views

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07:37 min

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January 26, 2024

DOI:

10.3791/65448-v

DOI:

10.3791/65448-v

•

07:37 min

January 26, 2024

•

3 Views

Ying V. Liu¹, Kang V. Li¹, Zhuolin Li¹, Yuchen Lu¹, Minda M. McNally¹, Edward P. Esposito¹, Kanza Aziz¹, Mandeep S. Singh^1,2

¹Wilmer Eye Institute,Johns Hopkins University School of Medicine, ²Department of Genetic Medicine,Johns Hopkins University School of Medicine

Summary

This protocol presents a transpupillary vision-guided trans-scleral approach to safely and precisely deliver subretinal cellular grafts, with a low rate of surgical complications, in mouse recipients with or without retinal degeneration.

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Transpupillary-Guided Trans-Scleral Transplantation of Subretinal Grafts in a Retinal Degeneration Mouse Model

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