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卵黄抗体に基づくサイトメトリービーズアッセイの標準化
JoVE Journal
Biyokimya
Author Produced
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JoVE Journal Biyokimya
Standardization of a Cytometric Bead Assay Based on Egg-Yolk Antibodies

卵黄抗体に基づくサイトメトリービーズアッセイの標準化

DOI:

10:23 min

May 19, 2023

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Bölümler

  • 00:04Introduction
  • 00:45Extraction of IgY from Egg Yolks
  • 02:27Saturation Curve of IgY Antibodies to Latex Beads
  • 04:02Flow Cytometry Assay Based on Latex Beads for Antigen Detection
  • 05:05Flow Cytometer Reading of the Samples and Sample Analysis
  • 07:33Results: Electrophoretic Profile of the IgY‐PfHRP2 Antibody
  • 09:29Conclusion

Özet

Otomatik Çeviri

このプロトコルは、抗原検出にIgY抗体を使用するアッセイ用のラテックスビーズを調製するための方法論を記載しています。

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