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Standardization of a Cytometric Bead Assay Based on Egg-Yolk Antibodies
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Standardization of a Cytometric Bead Assay Based on Egg-Yolk Antibodies

Standardization of a Cytometric Bead Assay Based on Egg-Yolk Antibodies

DOI:
10.3791/65123-v

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10:23 min

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May 19, 2023

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, , , , , , ,
1Programa de Pós-Graduação em Biotecnologia da Universidade Federal do Amazonas – UFAM, 2Instituto Leônidas e Maria Deane (ILMD),Fundação Oswaldo Cruz (FIOCRUZ), 3Programa de Pós-Graduação em Biologia da Interação Patógeno-Hospedeiro do Instituto Leônidas e Maria Deane (ILMD),Fundação Oswaldo Cruz (FIOCRUZ), 4Programa de Pós-graduação em Ciências Aplicadas à Hematologia – Universidade Estadual do Amazonas/Fundação Hospitalar de Hematologia e Hemoterapia do Amazonas, 5Programa de Pós-Graduação em Imunologia Básica e Aplicada, Instituto de Ciências Biológicas,Universidade Federal do Amazonas (UFAM)

Capítulos

  • 00:04Introduction
  • 00:45Extraction of IgY from Egg Yolks
  • 02:27Saturation Curve of IgY Antibodies to Latex Beads
  • 04:02Flow Cytometry Assay Based on Latex Beads for Antigen Detection
  • 05:05Flow Cytometer Reading of the Samples and Sample Analysis
  • 07:33Results: Electrophoretic Profile of the IgY‐PfHRP2 Antibody
  • 09:29Conclusion

Summary

Traducción Automática

Traducción Automática

This protocol describes a methodology for the preparation of latex beads for assays using IgY antibodies for antigen detection.

Tags

Cytometric Bead AssayEgg-yolk AntibodiesIgY AntibodiesFlow CytometryImmunoassayLatex BeadsPfHRP2StandardizationBradford AssayEDCNHS

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