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Ultrastructural Localization of Endogenous LC3 by On-Section Correlative Light-Electron Microscopy
JoVE Journal
Biyoloji
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JoVE Journal Biyoloji
Ultrastructural Localization of Endogenous LC3 by On-Section Correlative Light-Electron Microscopy

Ultrastructural Localization of Endogenous LC3 by On-Section Correlative Light-Electron Microscopy

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11:53 min

March 31, 2023

DOI:

11:53 min
March 31, 2023

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Here, we present a protocol for optimized on-section correlative light-electron microscopy based on endogenous, fluorescent labeling as a tool to investigate the localization of rare proteins in relation to cellular ultrastructure. The power of this approach is demonstrated by ultrastructural localization of endogenous LC3 in starved cells without Bafilomycin treatment.

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