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Ultrastructural Localization of Endogenous LC3 by On-Section Correlative Light-Electron Microscopy
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March 31, 2023
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Here, we present a protocol for optimized on-section correlative light-electron microscopy based on endogenous, fluorescent labeling as a tool to investigate the localization of rare proteins in relation to cellular ultrastructure. The power of this approach is demonstrated by ultrastructural localization of endogenous LC3 in starved cells without Bafilomycin treatment.
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van der Beek, J., Veenendaal, T., de Heus, C., van Dijk, S., ten Brink, C., Liv, N., Klumperman, J. Ultrastructural Localization of Endogenous LC3 by On-Section Correlative Light-Electron Microscopy. J. Vis. Exp. (193), e65067, doi:10.3791/65067 (2023).
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