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分离来自癌细胞系的低内毒素含量细胞外囊泡
JoVE Journal
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JoVE Journal İmmünoloji ve Enfeksiyon
Isolation of Low Endotoxin Content Extracellular Vesicles Derived from Cancer Cell Lines
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分离来自癌细胞系的低内毒素含量细胞外囊泡

800 Views

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06:28 min

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February 17, 2023

DOI:

10.3791/65062-v

DOI:
10.3791/65062-v

•

06:28 min
February 17, 2023

•

4 Views
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1Department of Clinical Immunology,Jagiellonian University Medical College, 2Doctoral School of Medical and Health Sciences,Jagiellonian University, 3Institute of Veterinary Sciences, University Center of Veterinary Medicine JU-AU,University of Agriculture in Kraków

Özet

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拟议的方案包括有关如何在从细胞培养上清液中分离细胞外囊泡以及如何正确评估它们期间避免内毒素污染的指南。

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Babula, A., Siemińska, I., Baj-Krzyworzeka, M. Isolation of Low Endotoxin Content Extracellular Vesicles Derived from Cancer Cell Lines. J. Vis. Exp. (192), e65062, doi:10.3791/65062 (2023).

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