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Isolierung von extrazellulären Vesikeln mit niedrigem Endotoxingehalt, die aus Krebszelllinien stammen
JoVE Journal
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JoVE Journal İmmünoloji ve Enfeksiyon
Isolation of Low Endotoxin Content Extracellular Vesicles Derived from Cancer Cell Lines
Please note that all translations are automatically generated. Click here for the English version.

Isolierung von extrazellulären Vesikeln mit niedrigem Endotoxingehalt, die aus Krebszelllinien stammen

800 Views

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06:28 min

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February 17, 2023

DOI:

10.3791/65062-v

DOI:
10.3791/65062-v

•

06:28 min
February 17, 2023

•

4 Views
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1Department of Clinical Immunology,Jagiellonian University Medical College, 2Doctoral School of Medical and Health Sciences,Jagiellonian University, 3Institute of Veterinary Sciences, University Center of Veterinary Medicine JU-AU,University of Agriculture in Kraków

Özet

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Das vorgeschlagene Protokoll enthält Richtlinien, wie eine Kontamination mit Endotoxin während der Isolierung von extrazellulären Vesikeln aus Zellkulturüberständen vermieden und richtig bewertet werden kann.

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Babula, A., Siemińska, I., Baj-Krzyworzeka, M. Isolation of Low Endotoxin Content Extracellular Vesicles Derived from Cancer Cell Lines. J. Vis. Exp. (192), e65062, doi:10.3791/65062 (2023).

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