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Scalable Transfection of Maize Mesophyll Protoplasts
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JoVE Journal Biyoloji
Scalable Transfection of Maize Mesophyll Protoplasts

Scalable Transfection of Maize Mesophyll Protoplasts

DOI:
10.3791/64991-v

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08:38 min

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June 23, 2023

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, , , ,
1Genome Sciences Department,Washington Üniversitesi

'Bölümler'

  • 00:04Introduction
  • 00:42Protoplast Isolation
  • 04:11Representative Results
  • 04:26PEG-Mediated Transfection
  • 06:57Representative Results
  • 07:39Conclusion

Özet

'Otomatik Çeviri'

'Otomatik Çeviri'

Here, we present a high-throughput protocol to transiently transfect millions of maize protoplasts for testing large libraries of plasmids in maize mesophyll cells.

'Etiketler'

MaizeMesophyll ProtoplastsScalable TransfectionCell Wall DigestionPEG TransformationHigh-efficiency TransformationDark-grown SeedlingsLeaf SectionsEnzyme SolutionVacuum InfiltrationTabletop ShakerCell StrainerCentrifugationProtoplast Washing

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