Here, we present a high-throughput protocol to transiently transfect millions of maize protoplasts for testing large libraries of plasmids in maize mesophyll cells.
Tonnies, J., Mueth, N. A., Gorjifard, S., Chu, J., Queitsch, C. Scalable Transfection of Maize Mesophyll Protoplasts. J. Vis. Exp. (196), e64991, doi:10.3791/64991 (2023).