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Monitoring On-Target Signaling Responses in Larval Zebrafish – Z-REX Unmasks Precise Mechanisms of Electrophilic Drugs and Metabolites
JoVE Journal
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Monitoring On-Target Signaling Responses in Larval Zebrafish – Z-REX Unmasks Precise Mechanisms of Electrophilic Drugs and Metabolites

Monitoring On-Target Signaling Responses in Larval Zebrafish – Z-REX Unmasks Precise Mechanisms of Electrophilic Drugs and Metabolites

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05:28 min

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June 02, 2023

DOI:

10.3791/64846-v

DOI:
10.3791/64846-v

•

05:28 min
June 02, 2023

•

6 Views
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1Swiss Federal Institute of Technology Lausanne (EPFL), 2Division of Genetics, Department of Medicine, Brigham and Women’s Hospital,Harvard Tıp Fakültesi, 3Department of Pharmacology and Toxicology, College of Pharmacy,Utah Üniversitesi, 4University of Lausanne (UNIL)

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Zebrafish targeting reactive electrophiles and oxidants (Z-REX) is a chemical biology-based method for the investigation of reactive small-molecule signaling. This technique can be applied to live fish of different developmental stages. Here, we couple standard assays in zebrafish with Z-REX for signaling pathway analysis.

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Cite this Article

Huang, K., Ly, P., Poganik, J. R., Parvez, S., Long, M. J. C., Aye, Y. Monitoring On-Target Signaling Responses in Larval Zebrafish - Z-REX Unmasks Precise Mechanisms of Electrophilic Drugs and Metabolites. J. Vis. Exp. (196), e64846, doi:10.3791/64846 (2023).

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