Dergi
/
Biyokimya
/
Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells
JoVE Journal
Biyokimya
This content is Free Access.
JoVE Journal Biyokimya
Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

DOI:
10.3791/64820-v

•

10:34 min

•

December 09, 2022

•

,
1Department of Microbiology,Harvard Tıp Fakültesi

Bölümler

  • 00:04Introduction
  • 00:36Gene‐Specific In Vitro DMS Modification
  • 02:49Gene‐Specific RT‐PCR of Modified RNA
  • 04:19Whole‐Genome DMS‐MaP Using Virus‐Infected Cells
  • 07:17Analysis of the Sequencing Data
  • 08:12Results: Probing RNA Structure with Dimethyl Sulfate Mutational Profiling and Sequencing
  • 10:00Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

The protocol provides instruction for modifying RNA with dimethyl sulfate for mutational profiling experiments. It includes in vitro and in vivo probing with two alternative library preparation methods.

Etiketler

RNA StructureDMS-MaPSequencingIn VitroIn CellsRNA Structure EnsembleBiological ResearchRefolding BufferDimethyl SulfateBeta-mercaptoethanolSpectrophotometerTGIRTComplementary DNAPCR

Article

Embed

ÇALMA LİSTESİNE EKLE

Usage Statistics

İlgili Videolar

Time-resolved ElectroSpray Ionization Hydrogen-deuterium Exchange Mass Spectrometry for Studying Protein Structure and Dynamics

Investigating Protein Sequence-structure-dynamics Relationships with Bio3D-web

Polysome Profiling in Leishmania, Human Cells and Mouse Testis

Characterization of Glycoproteins with the Immunoglobulin Fold by X-Ray Crystallography and Biophysical Techniques

Probing The Structure And Dynamics Of Nucleosomes Using Atomic Force Microscopy Imaging

Site-Directed Mutagenesis for In Vitro and In Vivo Experiments Exemplified with RNA Interactions in Escherichia Coli

Interactions with and Membrane Permeabilization of Brain Mitochondria by Amyloid Fibrils

Extremely Rapid and Specific Metabolic Labelling of RNA In Vivo with 4-Thiouracil (Ers4tU)

Rapid and Cost-Effective RNA Extraction of Rat Pancreatic Tissue

Phosphoproteomic Strategy for Profiling Osmotic Stress Signaling in Arabidopsis

Read Article