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Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells
JoVE 杂志
生物化学
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JoVE 杂志 生物化学
Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

2,652 Views

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10:34 min

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December 09, 2022

DOI:

10.3791/64820-v

DOI:
10.3791/64820-v

•

10:34 min
December 09, 2022

•

6 Views
,
1Department of Microbiology,Harvard Medical School

Tags

RNA StructureDMS-MaPSequencingIn VitroIn CellsRNA Structure EnsembleBiological ResearchRefolding BufferDimethyl SulfateBeta-mercaptoethanolSpectrophotometerTGIRTComplementary DNAPCR

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Dülk, S., Rouskin, S. Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells. J. Vis. Exp. (190), e64820, doi:10.3791/64820 (2022).

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