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从快速冷冻肝组织中分离细胞核用于单细胞多组学
JoVE Journal
Biyoloji
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JoVE Journal Biyoloji
Isolation of Nuclei from Flash-Frozen Liver Tissue for Single-Cell Multiomics
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从快速冷冻肝组织中分离细胞核用于单细胞多组学

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09:09 min

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December 09, 2022

DOI:

10.3791/64792-v

DOI:
10.3791/64792-v

•

09:09 min
December 09, 2022

•

75 Views
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1Helmholtz Pioneer Campus (HPC),Helmholtz Munich, 2Institute of Computational Biology, Computational Health Department,Helmholtz Munich, 3Division of Infectious Diseases and Tropical Medicine,Ludwig-Maximillian-Universität Klinikum, 4TUM School of Medicine,Technical University of Munich

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在这里，我们提出了一种从快速冷冻，存档的肝组织中分离细胞核的方案，用于单核RNA-seq，ATAC-seq和联合多组学（RNA-seq和ATAC-seq）。

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Cite this Article

Strzelecki, M., Yin, K., Talavera-López, C., Martinez-Jimenez, C. P. Isolation of Nuclei from Flash-Frozen Liver Tissue for Single-Cell Multiomics. J. Vis. Exp. (190), e64792, doi:10.3791/64792 (2022).

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