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Enrichment of Adult Mouse Dorsal Root Ganglia Neuron Cultures by Immunopanning
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February 24, 2023
DOI:
1Neuroscience and Mental Health Institute,Alberta Üniversitesi, 2Department of Medicine, Division of Neurology,Alberta Üniversitesi, 3Department of Medical Microbiology and Immunology,Alberta Üniversitesi, 4Department of Pharmacology,Alberta Üniversitesi, 5Department of Anesthesiology and Pain Medicine,Alberta Üniversitesi
Özet
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This paper describes an immunopanning protocol for adult mouse dorsal root ganglia. By adhering antibodies to culture plates, we can negatively select and remove non-neuronal cells. We show that the cultures are enriched for neurons using this protocol, allowing for an in-depth study of neuronal responses to manipulation.
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Maguire, A. D., Plemel, J. R., Kerr, B. J. Enrichment of Adult Mouse Dorsal Root Ganglia Neuron Cultures by Immunopanning. J. Vis. Exp. (192), e64603, doi:10.3791/64603 (2023).
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