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Enrichment of Adult Mouse Dorsal Root Ganglia Neuron Cultures by Immunopanning
Journal JoVE
Neurosciences
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Journal JoVE Neurosciences
Enrichment of Adult Mouse Dorsal Root Ganglia Neuron Cultures by Immunopanning

Enrichment of Adult Mouse Dorsal Root Ganglia Neuron Cultures by Immunopanning

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08:57 min

February 24, 2023

DOI:

08:57 min
February 24, 2023

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Summary

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This paper describes an immunopanning protocol for adult mouse dorsal root ganglia. By adhering antibodies to culture plates, we can negatively select and remove non-neuronal cells. We show that the cultures are enriched for neurons using this protocol, allowing for an in-depth study of neuronal responses to manipulation.

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