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Fast Colony Forming Unit Counting in 96-Well Plate Format Applied to the Drosophila Microbiome
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Fast Colony Forming Unit Counting in 96-Well Plate Format Applied to the Drosophila Microbiome

Fast Colony Forming Unit Counting in 96-Well Plate Format Applied to the Drosophila Microbiome

5,868 Views

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12:55 min

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January 13, 2023

DOI:

10.3791/64298-v

DOI:
10.3791/64298-v

•

12:55 min
January 13, 2023

•

35 Views
,
1Department of Embryology,Carnegie Institution for Science, 2Biyoloji Bölümü,Johns Hopkins Üniversitesi

Özet

Automatically generated

This method quantifies microbial abundance using a 96-well plate format to plate colony forming units (CFUs) and is applied to the Drosophila microbiome in whole fly homogenate samples. CFUs are counted with an automated image analysis software provided here.

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Colony Forming UnitCFUMicrobiomeDrosophilaHigh-throughputAutomated CountingGnotobiotic96-well PlateMikroakışkanlarMikrobiyolojiMicrobial Quantification

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Cite this Article

Dodge, R., Ludington, W. B. Fast Colony Forming Unit Counting in 96-Well Plate Format Applied to the Drosophila Microbiome. J. Vis. Exp. (191), e64298, doi:10.3791/64298 (2023).

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