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Fast Colony Forming Unit Counting in 96-Well Plate Format Applied to the Drosophila Microbiome
JoVE 杂志
生物学
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JoVE 杂志 生物学
Fast Colony Forming Unit Counting in 96-Well Plate Format Applied to the Drosophila Microbiome

Fast Colony Forming Unit Counting in 96-Well Plate Format Applied to the Drosophila Microbiome

5,896 Views

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12:55 min

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January 13, 2023

DOI:

10.3791/64298-v

DOI:
10.3791/64298-v

•

12:55 min
January 13, 2023

•

35 Views
,
1Department of Embryology,Carnegie Institution for Science, 2Department of Biology,Johns Hopkins University

Summary

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This method quantifies microbial abundance using a 96-well plate format to plate colony forming units (CFUs) and is applied to the Drosophila microbiome in whole fly homogenate samples. CFUs are counted with an automated image analysis software provided here.

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Colony Forming UnitCFUMicrobiomeDrosophilaHigh-throughputAutomated CountingGnotobiotic96-well PlateMicrofluidicsMicrobiologyMicrobial Quantification

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Cite this Article

Dodge, R., Ludington, W. B. Fast Colony Forming Unit Counting in 96-Well Plate Format Applied to the Drosophila Microbiome. J. Vis. Exp. (191), e64298, doi:10.3791/64298 (2023).

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