This protocol describes the isolation of double-negative thymocytes from the mouse thymus followed by retroviral transduction and co-culture on the delta-like 4-expressing bone marrow stromal cell line co-culture system (OP9-DL4) for further functional analysis.
Rodrigues, G. O. L., Li, W., Cramer, S. D., Winer, H. Y., Hsu, T. C., Gower, T., Hixon, J. A., Durum, S. K. Co-Culture and Transduction of Murine Thymocytes on Delta-Like 4-Expressing Stromal Cells to Study Oncogenes in T-Cell Leukemia. J. Vis. Exp. (196), e64271, doi:10.3791/64271 (2023).