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Co-Culture and Transduction of Murine Thymocytes on Delta-Like 4-Expressing Stromal Cells to Study Oncogenes in T-Cell Leukemia
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JoVE Journal がん研究
Co-Culture and Transduction of Murine Thymocytes on Delta-Like 4-Expressing Stromal Cells to Study Oncogenes in T-Cell Leukemia

Co-Culture and Transduction of Murine Thymocytes on Delta-Like 4-Expressing Stromal Cells to Study Oncogenes in T-Cell Leukemia

DOI:
10.3791/64271-v

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09:43 min

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June 09, 2023

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, , , , , , ,
1Cytokines and Immunity Section, Cancer Innovation Laboratory, National Cancer Institute,National Institutes of Health (NIH), 2Comparative Biomedical Scientist Training Program,NIH, 3Department of Pathobiology and Diagnostic Investigation, Veterinary Diagnostic Laboratory,Michigan State University, 4NCI-Frederick Laboratory Animal Sciences Program

章

  • 00:04Introduction
  • 00:47Seeding and Transfecting the Retrovirus-Producer Cells
  • 02:31Thymocyte Preparation and Depletion of CD4+ and CD8+ Cells
  • 04:53Harvesting Retrovirus and Transducing the Thymocytes
  • 07:13Results: Thymocyte Depletion and Thymocyte Retroviral Transduction Efficiency
  • 08:51Conclusion

概要

自動翻訳

自動翻訳

This protocol describes the isolation of double-negative thymocytes from the mouse thymus followed by retroviral transduction and co-culture on the delta-like 4-expressing bone marrow stromal cell line co-culture system (OP9-DL4) for further functional analysis.

タグ

Co-cultureTransductionMurine ThymocytesDelta-Like 4Stromal CellsT-cell LeukemiaRetroviral VectorsOP9 DL4In Vitro SystemT-cell DevelopmentCancerThymocyte SuspensionThymusPBSGlass SlidesCell Filtration

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