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猕猴的器官型视网膜外植体培养物
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JoVE Journal Nörobilim
Organotypic Retinal Explant Cultures from Macaque Monkey
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猕猴的器官型视网膜外植体培养物

DOI:
10.3791/64178-v

•

10:32 min

•

August 24, 2022

•

, , , , ,
1Yunnan Eye Institute & Key Laboratory of Yunnan Province, Yunnan Eye Disease Clinical Medical Center, Affiliated Hospital of Yunnan University,Yunnan University, 2Institute for Ophthalmic Research,Eberhard-Karls-Universität Tübingen

Bölümler

  • 00:04Introduction
  • 01:05Preparation of Retinal Explants
  • 03:08Primate Retinal Explant Culture
  • 03:36Fixing and Cryo‐Sectioning
  • 05:01Cyclic Guanosine Monophosphate (cGMP) Staining
  • 06:13TUNEL Staining
  • 07:58cGMP Staining Combined with TUNEL Staining
  • 08:40Results: Macaque Monkey Retinal Explant Culture
  • 09:20Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

从野生型猕猴获得的视网膜外植体在 体外培养。视网膜变性和cGMP-PKG信号通路使用PDE6抑制剂扎匹司特诱导。使用免疫荧光法验证不同浓度的扎普瑞斯特外植体中的cGMP积累。

Etiketler

Organotypic Retinal ExplantMacaque MonkeyRetinal DegenerationIn Vivo ConditionsAnimal Use ReductionRetinal DevelopmentRetinal Explant PreparationBacterial ContaminationRetinal Explant CultureDrug TreatmentCryo-sectioningParaformaldehyde Fixation

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