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マカクザルの有機型網膜外植片培養
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JoVE Journal Nörobilim
Organotypic Retinal Explant Cultures from Macaque Monkey
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マカクザルの有機型網膜外植片培養

1,100 Views

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10:32 min

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August 24, 2022

DOI:

10.3791/64178-v

DOI:
10.3791/64178-v

•

10:32 min
August 24, 2022

•

8 Views
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1Yunnan Eye Institute & Key Laboratory of Yunnan Province, Yunnan Eye Disease Clinical Medical Center, Affiliated Hospital of Yunnan University,Yunnan University, 2Institute for Ophthalmic Research,Eberhard-Karls-Universität Tübingen

Özet

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野生型マカクから得られた網膜外植体を インビトロで培養した。網膜変性およびcGMP−PKGシグナル伝達経路は、PDE6阻害剤ザプリナストを用いて誘導した。異なるザプリナスト濃度の外植体におけるcGMP蓄積は、免疫蛍光法を用いて検証された。

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Xu, W., Dong, Y., Li, Y., Hu, Z., Paquet-Durand, F., Jiao, K. Organotypic Retinal Explant Cultures from Macaque Monkey. J. Vis. Exp. (186), e64178, doi:10.3791/64178 (2022).

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