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Organotypic Retinal Explant Cultures from Macaque Monkey
JoVE 杂志
神经科学
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JoVE 杂志 神经科学
Organotypic Retinal Explant Cultures from Macaque Monkey

Organotypic Retinal Explant Cultures from Macaque Monkey

1,100 Views

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10:32 min

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August 24, 2022

DOI:

10.3791/64178-v

DOI:
10.3791/64178-v

•

10:32 min
August 24, 2022

•

8 Views
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1Yunnan Eye Institute & Key Laboratory of Yunnan Province, Yunnan Eye Disease Clinical Medical Center, Affiliated Hospital of Yunnan University,Yunnan University, 2Institute for Ophthalmic Research,Eberhard-Karls-Universität Tübingen

Summary

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Retinal explants obtained from wild-type macaques were cultured in vitro. Retinal degeneration and the cGMP-PKG signaling pathway was induced using the PDE6 inhibitor zaprinast. cGMP accumulation in the explants at different zaprinast concentrations was verified using immunofluorescence.

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Xu, W., Dong, Y., Li, Y., Hu, Z., Paquet-Durand, F., Jiao, K. Organotypic Retinal Explant Cultures from Macaque Monkey. J. Vis. Exp. (186), e64178, doi:10.3791/64178 (2022).

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