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剥离印迹技术：一种冷冻电镜样品制备方法，用于从多层或浓缩生物样品中分离单层
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JoVE Journal Biyokimya
The Peel-Blot Technique: A Cryo-EM Sample Preparation Method to Separate Single Layers From Multi-Layered or Concentrated Biological Samples
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剥离印迹技术：一种冷冻电镜样品制备方法，用于从多层或浓缩生物样品中分离单层

2,158 Views

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07:27 min

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June 29, 2022

DOI:

10.3791/64099-v

DOI:
10.3791/64099-v

•

07:27 min
June 29, 2022

•

2 Views
, ,
1School of Biological Sciences,Georgia Institute of Technology, 2Department of Structural Biology,Genentech, 3School of Chemistry and Biochemistry,Georgia Institute of Technology

Özet

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剥离-印迹技术是一种冷冻电镜网格制备方法，可将多层和浓缩的生物样品分离成单层，以减小厚度、提高样品浓度并促进图像处理。

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Johnson, M. C., Grant, A. J., Schmidt-Krey, I. The Peel-Blot Technique: A Cryo-EM Sample Preparation Method to Separate Single Layers From Multi-Layered or Concentrated Biological Samples. J. Vis. Exp. (184), e64099, doi:10.3791/64099 (2022).

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