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Reconstitutie van septineassemblage bij membranen om biofysische eigenschappen en functies te bestuderen
JoVE Journal
Biyokimya
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JoVE Journal Biyokimya
Reconstitution of Septin Assembly at Membranes to Study Biophysical Properties and Functions
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Reconstitutie van septineassemblage bij membranen om biofysische eigenschappen en functies te bestuderen

2,087 Views

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06:32 min

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July 28, 2022

DOI:

10.3791/64090-v

DOI:
10.3791/64090-v

•

06:32 min
July 28, 2022

•

2 Views
, , ,
1Department of Biochemistry and Biophysics,University of North Carolina at Chapel Hill, 2Curriculum in Genetics and Molecular Biology,University of North Carolina at Chapel Hill, 3Biyoloji Bölümü,University of North Carolina at Chapel Hill

Özet

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Celvrije reconstitutie is een belangrijk hulpmiddel geweest om de cytoskeletassemblage te begrijpen, en het werk in het afgelopen decennium heeft benaderingen vastgesteld om septinedynamica in minimale systemen te bestuderen. Hier worden drie complementaire methoden gepresenteerd om septineassemblage in verschillende membraancontexten te observeren: vlakke bilayers, sferische steunen en staafsteunen.

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Cite this Article

Curtis, B. N., Vogt, E. J. D., Cannon, K. S., Gladfelter, A. S. Reconstitution of Septin Assembly at Membranes to Study Biophysical Properties and Functions. J. Vis. Exp. (185), e64090, doi:10.3791/64090 (2022).

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