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Reconstitution of Septin Assembly at Membranes to Study Biophysical Properties and Functions
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JoVE Journal Biyokimya
Reconstitution of Septin Assembly at Membranes to Study Biophysical Properties and Functions

Reconstitution of Septin Assembly at Membranes to Study Biophysical Properties and Functions

2,087 Views

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06:32 min

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July 28, 2022

DOI:

10.3791/64090-v

DOI:
10.3791/64090-v

•

06:32 min
July 28, 2022

•

2 Views
, , ,
1Department of Biochemistry and Biophysics,University of North Carolina at Chapel Hill, 2Curriculum in Genetics and Molecular Biology,University of North Carolina at Chapel Hill, 3Biyoloji Bölümü,University of North Carolina at Chapel Hill

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Cell-free reconstitution has been a key tool to understand the cytoskeleton assembly, and work in the last decade has established approaches to study septin dynamics in minimal systems. Presented here are three complementary methods to observe septin assembly in different membrane contexts: planar bilayers, spherical supports, and rod supports.

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Curtis, B. N., Vogt, E. J. D., Cannon, K. S., Gladfelter, A. S. Reconstitution of Septin Assembly at Membranes to Study Biophysical Properties and Functions. J. Vis. Exp. (185), e64090, doi:10.3791/64090 (2022).

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