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Voorbereiding van nucleosoomkerndeeltjes gecomplexeerd met DNA-reparatiefactoren voor cryo-elektronenmicroscopie structurele bepaling
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JoVE Journal Biyokimya
Preparation of Nucleosome Core Particles Complexed with DNA Repair Factors for Cryo-Electron Microscopy Structural Determination
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Voorbereiding van nucleosoomkerndeeltjes gecomplexeerd met DNA-reparatiefactoren voor cryo-elektronenmicroscopie structurele bepaling

1,509 Views

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07:59 min

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August 17, 2022

DOI:

10.3791/64061-v

DOI:
10.3791/64061-v

•

07:59 min
August 17, 2022

•

5 Views
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1Genome Integrity and Structural Biology Laboratory,National Institute of Environmental Health Sciences, National Institutes of Health

Özet

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Dit protocol schetst in detail de voorbereiding van nucleosomale complexen met behulp van twee methoden voor monstervoorbereiding voor het bevriezen van TEM-roosters.

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NucleosomeDNA RepairCryo-electron MicroscopySample PreparationCross-linkingSize Exclusion ChromatographyBuffer ExchangeProtein Complex

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Rodriguez, Y., Butay, K. J., Sharma, K., Viverette, E., Wilson, S. H. Preparation of Nucleosome Core Particles Complexed with DNA Repair Factors for Cryo-Electron Microscopy Structural Determination. J. Vis. Exp. (186), e64061, doi:10.3791/64061 (2022).

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