Bu içeriği görüntülemek için JoVE aboneliği gereklidir. Oturum açın veya ücretsiz deneme sürümünü başlatın.
Preparation of Nucleosome Core Particles Complexed with DNA Repair Factors for Cryo-Electron Microscopy Structural Determination
1,520 Views
•
07:59 min
•
August 17, 2022
DOI:
Özet
Automatically generated
This protocol outlines in detail the preparation of nucleosomal complexes using two methods of sample preparation for freezing TEM grids.
Read Article
Cite this Article
Rodriguez, Y., Butay, K. J., Sharma, K., Viverette, E., Wilson, S. H. Preparation of Nucleosome Core Particles Complexed with DNA Repair Factors for Cryo-Electron Microscopy Structural Determination. J. Vis. Exp. (186), e64061, doi:10.3791/64061 (2022).
Copy