需要订阅 JoVE 才能查看此. 登录或开始免费试用。
Preparation of Nucleosome Core Particles Complexed with DNA Repair Factors for Cryo-Electron Microscopy Structural Determination
1,520 Views
•
07:59 min
•
August 17, 2022
DOI:
Summary
Automatically generated
This protocol outlines in detail the preparation of nucleosomal complexes using two methods of sample preparation for freezing TEM grids.
Read Article
Cite this Article
Rodriguez, Y., Butay, K. J., Sharma, K., Viverette, E., Wilson, S. H. Preparation of Nucleosome Core Particles Complexed with DNA Repair Factors for Cryo-Electron Microscopy Structural Determination. J. Vis. Exp. (186), e64061, doi:10.3791/64061 (2022).
Copy