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High-Speed Human Temporal Bone Sectioning for the Assessment of COVID-19-Associated Middle Ear Pathology
JoVE Journal
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JoVE Journal İmmünoloji ve Enfeksiyon
High-Speed Human Temporal Bone Sectioning for the Assessment of COVID-19-Associated Middle Ear Pathology

High-Speed Human Temporal Bone Sectioning for the Assessment of COVID-19-Associated Middle Ear Pathology

DOI:
10.3791/64012-v

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03:42 min

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May 18, 2022

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, , ,
1Department of Otolaryngology – Head & Neck Surgery,Johns Hopkins University School of Medicine, 2W. Harry Feinstone Department of Molecular Microbiology and Immunology,Johns Hopkins University Bloomberg School of Public Health, 3Patoloji Bölümü,Johns Hopkins University School of Medicine

Bölümler

  • 00:04Introduction
  • 00:47Temporal Bone Harvest
  • 01:40Tissue Fixation, Decalcification, and Sectioning
  • 02:27Results: Post-Processing Analyses of Middle Ear Tissue
  • 03:02Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

This article describes a technique for rapid human temporal bone sectioning that utilizes a microsaw with twin diamond blades to generate thin slices for rapid decalcification and analysis of temporal bone immunohistochemistry.

Etiketler

Temporal Bone SectioningCOVID-19Middle Ear PathologyRapid AssessmentOtopathologySARS-CoV2ACE2Transmembrane Protease SerineFurinImmunohistochemistry

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