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High-Speed Human Temporal Bone Sectioning for the Assessment of COVID-19-Associated Middle Ear Pathology
JoVE Journal
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JoVE Journal İmmünoloji ve Enfeksiyon
High-Speed Human Temporal Bone Sectioning for the Assessment of COVID-19-Associated Middle Ear Pathology

High-Speed Human Temporal Bone Sectioning for the Assessment of COVID-19-Associated Middle Ear Pathology

2,154 Views

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03:42 min

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May 18, 2022

DOI:

10.3791/64012-v

DOI:
10.3791/64012-v

•

03:42 min
May 18, 2022

•

2 Views
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1Department of Otolaryngology – Head & Neck Surgery,Johns Hopkins University School of Medicine, 2W. Harry Feinstone Department of Molecular Microbiology and Immunology,Johns Hopkins University Bloomberg School of Public Health, 3Patoloji Bölümü,Johns Hopkins University School of Medicine

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This article describes a technique for rapid human temporal bone sectioning that utilizes a microsaw with twin diamond blades to generate thin slices for rapid decalcification and analysis of temporal bone immunohistochemistry.

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Temporal Bone SectioningCOVID-19Middle Ear PathologyRapid AssessmentOtopathologySARS-CoV2ACE2Transmembrane Protease SerineFurinImmunohistochemistry

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Cite this Article

Andresen, N. S., Wood, M. K., Čiháková, D., Stewart, C. M. High-Speed Human Temporal Bone Sectioning for the Assessment of COVID-19-Associated Middle Ear Pathology. J. Vis. Exp. (183), e64012, doi:10.3791/64012 (2022).

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