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High-Speed Human Temporal Bone Sectioning for the Assessment of COVID-19-Associated Middle Ear Pathology
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
High-Speed Human Temporal Bone Sectioning for the Assessment of COVID-19-Associated Middle Ear Pathology

High-Speed Human Temporal Bone Sectioning for the Assessment of COVID-19-Associated Middle Ear Pathology

2,149 Views

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03:42 min

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May 18, 2022

DOI:

10.3791/64012-v

DOI:
10.3791/64012-v

•

03:42 min
May 18, 2022

•

2 Views
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1Department of Otolaryngology – Head & Neck Surgery,Johns Hopkins University School of Medicine, 2W. Harry Feinstone Department of Molecular Microbiology and Immunology,Johns Hopkins University Bloomberg School of Public Health, 3Department of Pathology,Johns Hopkins University School of Medicine

Summary

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This article describes a technique for rapid human temporal bone sectioning that utilizes a microsaw with twin diamond blades to generate thin slices for rapid decalcification and analysis of temporal bone immunohistochemistry.

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Temporal Bone SectioningCOVID-19Middle Ear PathologyRapid AssessmentOtopathologySARS-CoV2ACE2Transmembrane Protease SerineFurinImmunohistochemistry

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Cite this Article

Andresen, N. S., Wood, M. K., Čiháková, D., Stewart, C. M. High-Speed Human Temporal Bone Sectioning for the Assessment of COVID-19-Associated Middle Ear Pathology. J. Vis. Exp. (183), e64012, doi:10.3791/64012 (2022).

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