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Immunolabeling and Counting Ribbon Synapses in Young Adult and Aged Gerbil Cochleae
JoVE Journal
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JoVE Journal Nörobilim
Immunolabeling and Counting Ribbon Synapses in Young Adult and Aged Gerbil Cochleae

Immunolabeling and Counting Ribbon Synapses in Young Adult and Aged Gerbil Cochleae

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08:25 min

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April 21, 2022

DOI:

10.3791/63874-v

DOI:
10.3791/63874-v

•

08:25 min
April 21, 2022

•

5 Views
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1Cluster of Excellence “Hearing4all” and Research Centre Neurosensory Science, Department of Neuroscience, School of Medicine and Health Science,Carl von Ossietzky University Oldenburg

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A protocol for processing young adult and aged gerbil cochleae by immunolabeling the afferent synaptic structures and hair cells, quenching autofluorescence in aged tissue, dissecting and estimating the length of the cochleae, and quantifying the synapses in image stacks obtained with confocal imaging is presented.

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Steenken, F., Bovee, S., Köppl, C. Immunolabeling and Counting Ribbon Synapses in Young Adult and Aged Gerbil Cochleae. J. Vis. Exp. (182), e63874, doi:10.3791/63874 (2022).

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