Dergi
/
/
Single-Cell Proteomics Preparation for Mass Spectrometry Analysis Using Freeze-Heat Lysis and an Isobaric Carrier
JoVE Journal
Biyoloji
This content is Free Access.
JoVE Journal Biyoloji
Single-Cell Proteomics Preparation for Mass Spectrometry Analysis Using Freeze-Heat Lysis and an Isobaric Carrier

Single-Cell Proteomics Preparation for Mass Spectrometry Analysis Using Freeze-Heat Lysis and an Isobaric Carrier

3,419 Views

06:13 min

December 09, 2022

DOI:

06:13 min
December 09, 2022

10 Views
, ,

Özet

Automatically generated

In this protocol, we describe how to prepare mammalian cells for single-cell proteomics analysis via mass spectrometry using commercially available reagents and equipment, with options for both manual and automatic pipetting.

Read Article