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Single-Cell Proteomics Preparation for Mass Spectrometry Analysis Using Freeze-Heat Lysis and an Isobaric Carrier
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Biologia
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JoVE Journal Biologia
Single-Cell Proteomics Preparation for Mass Spectrometry Analysis Using Freeze-Heat Lysis and an Isobaric Carrier

Single-Cell Proteomics Preparation for Mass Spectrometry Analysis Using Freeze-Heat Lysis and an Isobaric Carrier

3,419 Views

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06:13 min

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December 09, 2022

DOI:

10.3791/63802-v

DOI:
10.3791/63802-v

•

06:13 min
December 09, 2022

•

10 Views
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1Department of Bioengineering,Northeastern University, 2Barnett Institute,Northeastern University, 3Department of Biology,Northeastern University

Summary

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In this protocol, we describe how to prepare mammalian cells for single-cell proteomics analysis via mass spectrometry using commercially available reagents and equipment, with options for both manual and automatic pipetting.

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Single-cell ProteomicsSCoPE2Mass SpectrometryFreeze-heat LysisIsobaric CarrierTrypsin DigestionTandem Mass TaggingLC-MS Analysis

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Cite this Article

Petelski, A. A., Slavov, N., Specht, H. Single-Cell Proteomics Preparation for Mass Spectrometry Analysis Using Freeze-Heat Lysis and an Isobaric Carrier. J. Vis. Exp. (190), e63802, doi:10.3791/63802 (2022).

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