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Mass-Rearing and Molecular Studies in Tortricidae Pest Insects
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Mass-Rearing and Molecular Studies in Tortricidae Pest Insects

Mass-Rearing and Molecular Studies in Tortricidae Pest Insects

2,545 Views

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06:22 min

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March 25, 2022

DOI:

10.3791/63737-v

DOI:
10.3791/63737-v

•

06:22 min
March 25, 2022

•

5 Views
, , ,
1Department of Applied Biological Science,Tokyo University of Agriculture and Technology

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The present protocol describes the rearing method of tortricid pest insects in the laboratories. The procedures to distinguish insects' sex and extract nucleic acids for high throughput sequencing are established using two tortricid pests.

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Arai, H., Ishitsubo, Y., Nakai, M., Inoue, M. N. Mass-Rearing and Molecular Studies in Tortricidae Pest Insects. J. Vis. Exp. (181), e63737, doi:10.3791/63737 (2022).

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