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环境科学
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Mass-Rearing and Molecular Studies in Tortricidae Pest Insects
JoVE 杂志
环境科学
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JoVE 杂志 环境科学
Mass-Rearing and Molecular Studies in Tortricidae Pest Insects

Mass-Rearing and Molecular Studies in Tortricidae Pest Insects

DOI:
10.3791/63737-v

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06:22 min

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March 25, 2022

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, , ,
1Department of Applied Biological Science,Tokyo University of Agriculture and Technology

Chapters

  • 00:05Introduction
  • 00:42Insect Collection and Mass Rearing
  • 01:32Separation of Eggs and Pharate Larvae from Egg Masses for Fixation, Permeabilization, Staining, and DNA and RNA Extraction from Pharate Larvae
  • 04:35Results: Morphological Observation of Embryos and Nucleic Acid Extraction
  • 05:52Conclusion

Summary

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The present protocol describes the rearing method of tortricid pest insects in the laboratories. The procedures to distinguish insects' sex and extract nucleic acids for high throughput sequencing are established using two tortricid pests.

Tags

TortricidaeMass RearingMolecular StudiesPest InsectsInsect EggsArtificial DietEgg MassSodium HypochloritePotassium HydroxideHeptaneParaformaldehydeMethanolEthanolDAPILactic AcidAcetic AcidLarval Dissection

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