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离体 啮齿动物三叉血管系统中降钙素基因相关肽的释放
JoVE Journal
Nörobilim
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JoVE Journal Nörobilim
Ex Vivo Release of Calcitonin Gene-Related Peptide from the Trigeminovascular System in Rodents
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离体 啮齿动物三叉血管系统中降钙素基因相关肽的释放

DOI:
10.3791/63723-v

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08:39 min

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May 16, 2022

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, , ,
1Danish Headache Center, Department of Neurology,Rigshospitalet, University of Copenhagen, 2EHESP, Irset (Institut de Recherche en Santé, Environnement et Travail),Univ Rennes, Inserm, 3Department of Biology, Section of Cell Biology and Physiology,University of Copenhagen

Bölümler

  • 00:04Introduction
  • 00:43Preparing Rat Tissue
  • 02:05Preparing Mouse Tissue
  • 03:16Washing and Preparation of Rat and Mouse Tissue
  • 04:21Preparation of Sample Collecting Tubes and Test Solutions
  • 04:46Determining Basal CGRP Release Levels
  • 05:26Performing Concentration‐Response Stimulation, Positive Control for the Experiment, and Measurement of CGRP Contractions
  • 06:29Results: Capsaicin‐Induced and TRPA1‐Dependent CGRP Release from Rat and Mouse Tissue
  • 08:04Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

本协议描述了 离体 降钙素基因相关肽（CGRP）释放模型以及量化药理学药物对啮齿动物三叉神经血管系统释放的CGRP量的影响的策略。

Etiketler

Ex VivoCalcitonin Gene-related PeptideTrigeminovascular SystemRodentsTrigeminal Vascular SystemCGRP ReleaseTrigeminal NerveTrigeminal GanglionTrigeminal Nucleus CaudalisBrain DissectionBrainstemCerebellumCranium

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