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Ex Vivo Release of Calcitonin Gene-Related Peptide from the Trigeminovascular System in Rodents
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Ex Vivo Release of Calcitonin Gene-Related Peptide from the Trigeminovascular System in Rodents

Ex Vivo Release of Calcitonin Gene-Related Peptide from the Trigeminovascular System in Rodents

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1,957 Views

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08:39 min

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May 16, 2022

DOI:

10.3791/63723-v

DOI:
10.3791/63723-v

•

08:39 min
May 16, 2022

•

9 Views
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1Danish Headache Center, Department of Neurology,Rigshospitalet, University of Copenhagen, 2EHESP, Irset (Institut de Recherche en Santé, Environnement et Travail),Univ Rennes, Inserm, 3Department of Biology, Section of Cell Biology and Physiology,University of Copenhagen

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The present protocol describes the ex vivo calcitonin gene-related peptide (CGRP) release model and the strategy to quantify the effect of pharmacological agents on the amount of CGRP released from the trigeminovascular system in rodents.

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Rasmussen, R. H., Jansen-Olesen, I., Kristensen, D. M., Christensen, S. L. Ex Vivo Release of Calcitonin Gene-Related Peptide from the Trigeminovascular System in Rodents. J. Vis. Exp. (183), e63723, doi:10.3791/63723 (2022).

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