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膜関連高分子ダイナミクスを特徴付けるための質量感受性粒子追跡
JoVE Journal
Biyokimya
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JoVE Journal Biyokimya
Mass-Sensitive Particle Tracking to Characterize Membrane-Associated Macromolecule Dynamics
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膜関連高分子ダイナミクスを特徴付けるための質量感受性粒子追跡

3,559 Views

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13:30 min

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February 18, 2022

DOI:

10.3791/63583-v

DOI:
10.3791/63583-v

•

13:30 min
February 18, 2022

•

21 Views
, , ,
1Department of Cellular and Molecular Biophysics,Max Planck Institute of Biochemistry, 2Fizik Bölümü,Technical University Munich, 3Department of Cellular Physiology, Biomedical Center (BMC),Ludwig-Maximilians-Universität München

Özet

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このプロトコルは、iSCATベースの画像処理と単粒子追跡アプローチを記述し、脂質膜と相互作用する高分子の分子量と拡散挙動の同時調査を可能にします。サンプル調製、質量コントラスト変換、ムービー取得、および後処理に関するステップバイステップの手順が、潜在的な落とし穴を防ぐための指示とともに提供されます。

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Steiert, F., Heermann, T., Hundt, N., Schwille, P. Mass-Sensitive Particle Tracking to Characterize Membrane-Associated Macromolecule Dynamics. J. Vis. Exp. (180), e63583, doi:10.3791/63583 (2022).

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