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Mass-Sensitive Particle Tracking to Characterize Membrane-Associated Macromolecule Dynamics
JoVE Journal
Biochemie
This content is Free Access.
JoVE Journal Biochemie
Mass-Sensitive Particle Tracking to Characterize Membrane-Associated Macromolecule Dynamics

Mass-Sensitive Particle Tracking to Characterize Membrane-Associated Macromolecule Dynamics

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13:30 min

February 18, 2022

DOI:

13:30 min
February 18, 2022

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Summary

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This protocol describes an iSCAT-based image processing and single-particle tracking approach that enables the simultaneous investigation of the molecular mass and the diffusive behavior of macromolecules interacting with lipid membranes. Step-by-step instructions for sample preparation, mass-to-contrast conversion, movie acquisition, and post-processing are provided alongside directions to prevent potential pitfalls.

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