Journal
/
/
Mass-Sensitive Particle Tracking to Characterize Membrane-Associated Macromolecule Dynamics
JoVE 杂志
生物化学
This content is Free Access.
JoVE 杂志 生物化学
Mass-Sensitive Particle Tracking to Characterize Membrane-Associated Macromolecule Dynamics

Mass-Sensitive Particle Tracking to Characterize Membrane-Associated Macromolecule Dynamics

3,559 Views

13:30 min

February 18, 2022

DOI:

13:30 min
February 18, 2022

21 Views
, , ,

Summary

Automatically generated

This protocol describes an iSCAT-based image processing and single-particle tracking approach that enables the simultaneous investigation of the molecular mass and the diffusive behavior of macromolecules interacting with lipid membranes. Step-by-step instructions for sample preparation, mass-to-contrast conversion, movie acquisition, and post-processing are provided alongside directions to prevent potential pitfalls.

Read Article