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Mass-Sensitive Particle Tracking to Characterize Membrane-Associated Macromolecule Dynamics
JoVE 杂志
生物化学
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JoVE 杂志 生物化学
Mass-Sensitive Particle Tracking to Characterize Membrane-Associated Macromolecule Dynamics

Mass-Sensitive Particle Tracking to Characterize Membrane-Associated Macromolecule Dynamics

3,650 Views

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13:30 min

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February 18, 2022

DOI:

10.3791/63583-v

DOI:
10.3791/63583-v

•

13:30 min
February 18, 2022

•

21 Views
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1Department of Cellular and Molecular Biophysics,Max Planck Institute of Biochemistry, 2Department of Physics,Technical University Munich, 3Department of Cellular Physiology, Biomedical Center (BMC),Ludwig-Maximilians-Universität München

Summary

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This protocol describes an iSCAT-based image processing and single-particle tracking approach that enables the simultaneous investigation of the molecular mass and the diffusive behavior of macromolecules interacting with lipid membranes. Step-by-step instructions for sample preparation, mass-to-contrast conversion, movie acquisition, and post-processing are provided alongside directions to prevent potential pitfalls.

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Steiert, F., Heermann, T., Hundt, N., Schwille, P. Mass-Sensitive Particle Tracking to Characterize Membrane-Associated Macromolecule Dynamics. J. Vis. Exp. (180), e63583, doi:10.3791/63583 (2022).

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