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质量敏感颗粒跟踪，用于表征膜相关的高分子动力学
JoVE Journal
Biyokimya
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JoVE Journal Biyokimya
Mass-Sensitive Particle Tracking to Characterize Membrane-Associated Macromolecule Dynamics
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质量敏感颗粒跟踪，用于表征膜相关的高分子动力学

3,559 Views

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13:30 min

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February 18, 2022

DOI:

10.3791/63583-v

DOI:
10.3791/63583-v

•

13:30 min
February 18, 2022

•

21 Views
, , ,
1Department of Cellular and Molecular Biophysics,Max Planck Institute of Biochemistry, 2Fizik Bölümü,Technical University Munich, 3Department of Cellular Physiology, Biomedical Center (BMC),Ludwig-Maximilians-Universität München

Özet

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该协议描述了一种基于iSCAT的图像处理和单粒子跟踪方法，可以同时研究与脂质膜相互作用的大分子的分子质量和扩散行为。在提供样品制备、质量到对比度转换、电影采集和后处理的分步说明的同时，还提供了相应的说明，以防止潜在的陷阱。

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Steiert, F., Heermann, T., Hundt, N., Schwille, P. Mass-Sensitive Particle Tracking to Characterize Membrane-Associated Macromolecule Dynamics. J. Vis. Exp. (180), e63583, doi:10.3791/63583 (2022).

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