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测量 果蝇 光感受器中光色素水平的电生理学方法
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JoVE Journal Nörobilim
Electrophysiological Methods for Measuring Photopigment Levels in Drosophila Photoreceptors
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测量 果蝇 光感受器中光色素水平的电生理学方法

DOI:
10.3791/63514-v

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08:09 min

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June 02, 2022

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, ,
1Department of Medical Neurobiology, Faculty of Medicine and the Edmond and Lily Safra Center for Brain Sciences (ELSC),Hebrew University

Bölümler

  • 00:04Introduction
  • 00:50Measuring the Prolonged Depolarizing Afterpotential (PDA) and the Early Receptor Potential (ERP) Using the Electroretinogram (ERG)
  • 05:28Results: Detecting Abnormalities in Photopigment Biogenesis by Measuring Photopigment Levels in Drosophila Using the Electroretinogram
  • 07:13Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

我们提出了一种电生理学表征双稳定光色素的方案：（i）利用光子吸收后光色素分子内的电荷位移及其在光感受器中的大量电荷，以及（ii）利用视紫红质和偏视紫红质光色素状态的吸收光谱差异。这些方案可用于筛选影响双稳定光色素系统的突变。

Etiketler

ElectrophysiologicalPhotopigmentDrosophilaPhotoreceptorsPhototransductionGenetic ScreenIn VivoElektrofizyolojiRecording ElectrodeGround ElectrodeClampexAmplifierMaster-8Xenon LampShutter ControllerGlass CapillaryRinger SolutionPlatinum-iridium FilamentWaxAnesthetized FliesFly HolderFaraday CageMagnet BlockLight Guide

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